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One Step qRT-PCR

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KAPA SYBR® FAST One-Step qRT-PCR Kits

Containing the first DNA polymerase evolved for real-time PCR.

KAPA SYBR® FAST One-Step qRT-PCR Kits contain M-MuLV Reverse Transcriptase, RNase Inhibitor and a novel DNA Polymerase engineered via molecular evolution. The kit is optimized for rapid one-step, one-tube RNA quantification. Reduce experimental variation and contamination with a convenient one-step qRT-PCR protocol.

KAPA SYBR® FAST One-Step qRT-PCR Kits are designed to perform optimally in stringent qRT-PCR reaction conditions, exhibiting dramatic improvements in sensitivity, specificity, reaction efficiency, and fluorescence.

Kits are ideally suited for:

  • Gene expression analysis
  • Microarray validation
  • Gene knockdown validation
  • RNAi and miRNA research

Ordering Information

 

CodeDescriptionKit Contents  KK4650KAPA SYBR® FAST One-Step qRT-PCR Universal (1 mL)For 100 x 20 uL reactions. Kits include 50X KAPA RT Mix (40 µL) and 10 mM dUTP (40 µL). Supplied with separate 50X ROX High and 50X ROX Low (200 µL each). KK4660KAPA SYBR® FAST One-Step qRT-PCR ABI Prism (1 mL)For 100 x 20 uL reactions. Kits include 50X KAPA RT Mix (40 µL) and 10 mM dUTP (40 µL). ROX is included in the master mix at an optimal concentration for most ABI qPCR cyclers KK4670KAPA SYBR® FAST One-Step qRT-PCR Bio-Rad iCycler (1 mL)For 100 x 20 uL reactions. Kits include 50X KAPA RT Mix (40 µL) and 10 mM dUTP (40 µL). Fluorescein is included in the master mix at an optimal concentration for most Bio-Rad qPCR cyclers. 

KK4680KAPA SYBR® FAST One-Step qRT-PCR Roche LightCycler® 480 (1 mL)For 100 x 20 uL reactions. Kits include 50X KAPA RT Mix (40 µL) and 10 mM dUTP (40 µL). Optimized for use with the LightCycler 480.

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