Long Range PCR

KAPA Long Range PCR Kits

Amplify long templates up to 20 kb with extreme sensitivity and increased fidelity.

The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an engineered archaeal (B-family) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long range and/or sensitive PCR.

KAPA Long Range DNA Polymerase possesses 3x – 4x higher fidelity than Taq polymerase. The majority of PCR products from a KAPA Long Range HotStart DNA Polymerase reaction have a single deoxyadenosine added to the 3’ ends of the amplicon and can be used for ligation into single 3’-T -overhang cloning sites.

The KAPA Long Range PCR system is ideally suited for:

• PCR amplification of long targets up to 20 kb and/or PCR using low amounts of template DNA.
• Standard short- and mid-range PCR amplification.
• Production of PCR products to be used for ligation into 3’-T-overhang cloning vectors.
• Targeted sample enrichment for next-generation DNA sequencing.

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